Extraction of caffeine from tea pdf. This process, known as liquid-liquid extraction, is a basic operation that should be mastered in the organic chemistry laboratory. Extraction device. 4. "3c-NJy$ I I A 3. However, the solubility of the individual catechins varies and depends on the extraction Jan 2, 2018 · After all our main stress is on the presence of caffeine in xanthene beverages and so in this project we will study and observe the quantity of caffeine varying in different samples of tea leaves. Subila, M. Flowchart - Prepare a flowchart of procedures AND show the location of the caffeine throughout each step of the experiment in the flow chart. Extraction step: Sublimation step: Take 5 tea bags and record the weight of these tea bags. Extraction of the Caffeine from the Tea When the tea has cooled to room temperature, add 3 mL of methylene chloride (CH 2 Cl 2 ) to each centrifuge tube. S. Caffeine is a bitter, white crystalline alkaloid present in around 60 plant species, most commonly coffee beans (Figure 3), tea leaves and kola nuts. It works by stimulating the brain. Mar 25, 2024 · The caffeine can then be extracted from the water by methylene chloride in almost pure form. The melting point of caffeine that was obtained in this experiment was 224° C. Procedure Add about 10. The technicians are divided into two groups. Place 50 mL hot water and 2. The extraction process was repeated 3 more times. Repeat steps a) through e) twice. Turn the heat off and place 3 tea bags of black tea in the hot water. txt) or view presentation slides online. 4 million tonnes by 2025. Oct 2, 2018 · Caffeine (1,3,7-trimethylxanthine) is a purine alkaloid present in popular drinks such as coffee and tea. Water-soluble components in the tealeaves or coffee beans are being Sep 7, 2023 · For reference, a 12 ounce can of a caffeinated soft drink typically contains 30 to 40 milligrams of caffeine, an 8-ounce cup of green or black tea 30-50 milligrams, and an 8-ounce cup of coffee Aug 14, 2017 · Here are 9 side effects of too much caffeine. 5 grams of sodium carbonate (Na 2 CO 3 Discussion: The basic property of caffeine comes from the lone pair of electrons found around the nitrogen. Lab Partner: Sean Wilson. Consuming a large amount of these types of tea could cause some health problems. 4g of calcium carbonate were poured in a 150mL beaker and boiled gently for 15 minutes. 3 million tonnes in 2020 to 6. Microwave assisted extraction was applied to extract tea components from green tea then i) conventional liquid-liquid extraction or ii) supercritical carbon dioxide fluid extraction (SFE) method was charged with the effective separation of caffeine and catechins. Chocolate also contains caffeine. October 6, 2013. A typical catechin profile in an extract from green tea leaves comprises 10–15% EGCG, 6–10% EGC, 2–3% ECG, and 2% EC [27]. Hot plate. After boiling for 15 minutes, carefully remove the tea bags and place them in a clean beaker to cool off. Quantity of caffeine in Red label tea is 60mg. Jun 21, 2016 · The purpose of this experiment was to perform a liquid-liquid extraction method to extract the caffeine from the tea bags that were provided, and then recrystallize the caffeine. 1n the Cola drinks present experiment we are going to use tea leaves as the source of caffeine. was used in the sample preparation step using 2. Isolation of Caffeine from Tea Hayley Williams, willi553@go. Gönül Serdar Ezgi Demir Serhat Bayrak M. Chemistry. 98 million metric tonnes during 2021–21. That is why tea removes fatigue, tiredness and headache. After isolating and evaporating the crude caffeine, 0. [13] Caffeine may affect mental states such as increasing alertness and attention, reducing anxiety, and improving mood. 6: Caffeine Isn't Healthy for Kids As many as 75% of kids have some caffeine every day. Since we are not interested in Caffeine is the principle stimulant found in tea and coffee, and it can account for up to approximately 5% of the mass of the tea leaves. Jan 7, 2004 · Request PDF | Solid extraction of caffeine and theophylline from green tea by molecular imprinted polymers | This paper involves a feasibility study on using molecular imprinted polymers as the The extraction and gravimetric determination of caffeine from finely cut tea leaves is chosen as the “test”. Pure caffeine (trimethylxanthine) occurs as a white powder or as silky needles, which melt at 238 °C (460 °F); it Jan 9, 2024 · In this experiment you will extract caffeine from tea leaves and use sublimation to purify the caffeine. In this study, considering the co-extraction of the valuable bioactive catechins, the concentrations of EC, ECG, EGC, EGCG and Jun 1, 2006 · The effect of different extraction set-ups that influence the extraction efficiency of catechins and caffeine from green tea leaves (variety Fanning Belas, China) were studied using different Caffeine solution will be isolated from tea by dichloromethane solution and be purified by calcium chloride beads. 9 to 110. In this experiment, you will carry out the following steps. Caffeine is found in many beverages such as coffee Caffeine can increase blood pressure and cause vasoconstriction. Feb 1, 2003 · From the points of extraction time, the extraction efficiency and the percentages of tea polyphenols or tea caffeine in extracts, MAE was more effective than the conventional extraction methods studied. Green tea — about 20 mg of caffeine. Caffeine is also available in prescription and non-prescription medications. At the Caffeine Lab 1 Isolation of Caffeine from tea In this experiment, caffeine will be extracted from tea leaves (where it is about 5% present) using hot water. 73, and in black tea was in the range from 10. The SFE of caffeine was carried out at different pressure 2. It acts as a stimulant for central nervous system and skeletal muscles. This is essentially the same procedure used to decaffeinate drinks such as coffee and tea. Black Tea was generally Apr 1, 2022 · Comparative Evaluation of caffeine extraction in waste tea as a potential low-cost source relative to dry tea in different solvents. The list below shows the amount of caffeine in a 7 oz. 1. Caffeine is soluble in water at approximately 2 mg/ml at 25°C, 180 mg/ml at 80° C, and 670 mg/ml at 100°C. 01 AVT 0. Caffeine, nitrogenous organic compound of the alkaloid group, substances that have marked physiological effects. 1398 g Percentage yield = Mass of crude caffeine / mass of tea leaves x 100% = 0. Now place the 5 tea bags in this beaker. Melting point apparatus. Put 10g tea powder into the folded filter paper sleeve, and then put the sleeve into the Soxhlet extractor. 2016. It may have neurological benefits in some people and act as an antidepressant. The Crude Caffeine was then evaporated using a hot plate in the hood, leaving behind Mar 27, 2014 · Standard tea bags contain 2. Caffeine and other purine alkaloids, including theobromine and theophylline, play a major role in the long-term popularity of non-alcoholic beverages and foods such as coffee, tea, cocoa, chocolate and a variety of soft drinks. Jun 1, 2006 · The effect of different extraction set-ups that influence the extraction efficiency of catechins and caffeine from green tea leaves (variety Fanning Belas, China) were studied using different aqueous and pure solvents (acetone, ethanol, methanol, acetonitrile, water), different temperatures (60, 80, 95 and 100 °C) and times (5–240 min). Tea bags are used as the source of caffeine for this experiment. the procedures. Quantity of caffeine in green label tea is 45mg. Caffeine is a drug that stimulates (increases the activity of) your brain and nervous system. According to a 2020 study in the United States, coffee is the major source of caffeine intake in middle-aged adults, while soft drinks and tea are the major sources Jan 1, 2017 · Caffeine Extraction from Tea and Coffee. 5 grams calcium carbonate in a 250 mL beaker. 008g and the percentage recovered of caffeine in the tea bag was 0. One study found that kids between the ages of 8 and 12 have as much caffeine as you'd find Jun 30, 2013 · The extraction of tea polyphenols and tea caffeine with MAE for 4 min (30 and 4%) were higher than those of extraction at room temperature for 20 h, ultrasonic extraction for 90 min and heat Dec 18, 2023 · Furthermore, caffeine-containing beverages like tea and coffee are socially acceptable and widely used. The step-by-step process of liquid-liquid extraction of caffeine from the samples, namely coffee, tea, and soda, were shown in Figure 1. Tea bags are used to extract caffeine from tea through a multi-step liquid-liquid extraction and purification process. , 2010; Khan In this experiment we will isolate caffeine from tea. This drug is most commonly sourced from the coffee bean but can also be found naturally occurring in certain types of tea and cacao beans, and it is also an additive to soda and energy drinks. the extraction of caffeine from tea ppt - Free download as Powerpoint Presentation (. Separating funnel. Caffeine is known to have a bitter taste and is used by Barq’s as a flavoring to add a sharp bitter taste to their root beer. /sample of 50 gm. Energy drinks often have more caffeine and sugar than soft drinks. This method dissolves one or more (components) compounds into an appropriate solvent. The mass of the crystal caffeine will be measured to calculate the percent New Approaches for Effective Microwave Assisted Extraction of Caffeine and Catechins from Green Tea. Environmental Science, Chemistry. The major constituent of tea is cellulose Sep 18, 2023 · Procedure for Caffeine Extraction (to be performed in pairs) Place about 100 mL of deionized water into a beaker (250 mL) and bring to a gentle boil on a hot plate. Using caffeine as a raw material, we can prepare an Anacin drug with several medicinal uses. Theory: The technique used to separate an organic compound from a mixture of compounds is called Extraction. In the case of Caffeine extraction from tea powder or leaves, the solubility of caffeine in water is approximately 20g/l at 25°C, 600g/l at 80°C, and 670g/l at 100°C. Read the full fact sheet. Other natural sources of caffeine include yerba mate, guarana berries, guayusa, and the yaupon holly. The side effects of caffeine include a short-lived rise in heart rate and blood pressure, heart palpitations, headache, increased urine output, nervousness, gastrointestinal Tea bags. 4318 g Weight of crude caffeine 0. Caffeine is a minor constituent of tea, coffee, and other natural plant materials. Six teabags were steeped for 2 minutes before sodium carbonate was added. The primary goal of caffeine consumption Experiment 2 – Isolation and Sublimation of Caffeine from Tea Leaves. Caffeine is known to increase alertness. Some known mechanisms of action explain the effects of caffeine. The effect of different parameters such as high hyd… The experiment of extraction of caffeine from tea is very professional which is time consuming and the extractor is used is poisonous; Caffeine is bitter, white crystalline xanthine alkaloid and stimulant drug. 10. . 35%. Press the tea bags with a clean cork to express as much liquid as possible without breaking the bag and transfer this liquid to the 50 ml Erlenmeyer flask also. Caffeine is an alkaloid occurring naturally in some 60 plant species, of which cocoa beans, kola nuts, tea leaves and coffee beans are the most well-known. 02 Palat 0. Scribd is the world's largest social reading and publishing site. The crude caffeine is further purified through sublimation, where it is heated until vaporizing Content of caffeine in the green tea was in the range from 33. Abstract: Caffeine was extracted from tea by the use of solid-liquid and liquid-liquid. Take 500 ml beaker add 200 ml of distilled water to it. 02 Kannan Devan 0. Caffeine can also produce negative allelopathic effects that prevent the growth of surrounding plants. , 2009). 8. Caffeine is found naturally in foods and beverages such as coffee, tea, colas, energy and chocolate. Sep 28, 2023 · Caffeine Myth No. January 2017. James Chickos, David Garin, and Val erian D'Souza. Since caffeine is more soluble in the organic solvent dichloromethane (CH 2 Cl 2 Black tea contains 25-110 mg of caffeine per cup. University of Missouri–St. extractions. 60 -75 mg of caffeine is present the number of other brewages listed in table-01. Then the dichloromethane/caffeine solution will be put in the rotary evaporator to evaporate the solution, leaving crystal caffeine as the final product. ppt / . Students play the role of laboratory technicians and Weight of china dish with precipitate. May 13, 2024 · After evaporation the combined chloroform extract gives caffeine as a white colour material and by using alcohol again it is re crystallize. Jan 1, 2024 · The worldwide consumption of coffee increased from 9. 5 times more effective than only water for extraction of The caffeine is determined by using UV-Visible spectroscopy method using dichloromethane as an extracting solvent at 273nm wavelength. 85 million tonnes during 2019–20 to 9. Sökmen. Navis. Process Flow Diagram for the Isolation of Caffeine through LiquidLiquid Extraction IV. You will then perform a liquid-liquid extraction of the tea solution with an organic solvent, dichloromethane, to separate the caffeine from the basic tea solution and away from the tannins, gallic acid and the remainder of the tea components. 00 +/- 0. Caffeine products sold in very concentrated or pure forms are a health concern. 45gms. Dry the combined dichloromethane solutions with anhydrous Sodium sulfite. 5 g of the sample (Komes et. Heat the beaker on a hot plate (setting. The amount of caffeine in different liquids varied in one cup of tea. Extraction process selectively dissolves one or more of the mixture compounds into a suitable solvent. [14] A moderate caffeine intake of less than 6 cups of coffee per day has been associated with a lower risk of depression and suicide. Quantity of caffeine in yellow label tea is 55mg. Caffeine is a naturally occurring chemical stimulant found in the leaves, seeds and fruits of many plant species of a group of compounds called trimethyl xanthine. After treatment of 10% H 2 SO 4 caffeine Apr 3, 2024 · Experiment #3 Extraction of Caffeine from Tea Charlotte Lee Lab Partner - Rachel Lin Teaching Assistant - Yifan Liu Date Performed: February 20, 2024 Date Due: Feb 27, 2024 Charlotte Lee 2 Experimental Objective To isolate caffeine from tea using liquid Jul 14, 2016 · PDF | This study was designed to develop an effective microwave assisted extraction (MAE) method for extraction of caffeine and catechins from green tea | Find, read and cite all the research May 20, 2024 · e. 성철 홍. Oct 1, 2015 · Thus, extraction time was set to 20 min in order to proceed with the study of (i) the effect of temperature and (ii) the use of ethyl lactate + water mixtures on the extraction of caffeine from green tea leaves. Aug 22, 2023 · Caffeine is a central nervous system stimulant. 03 Eastern Eastea 0. In soxhlet extractor, by using ethanol the coarsely powdered tea leaves are extracted. 32 to 63 (mg/g). This study was designed to develop an effective microwave assisted extraction (MAE) method for extraction of caffeine and catechins from green tea samples. Caffeine extract caffeine from coffee. 1398 g / 20. 065 g caffeine was obtained. A pure product of . 8 Jun 8, 2023 · Caffeine is a naturally occurring central nervous system (CNS) stimulant of the methylxanthine class and is the most widely taken psychoactive stimulant globally. The highest content of caffeine was detected in the green tea from the Slovenian market, and in the black tea from Croatian market. 0110 g Weight of empty round bottom flask 113. 2 Physical properties of tea catechins Catechins are colourless and are soluble in water and polar organic solvents [1, 6]. 05gms. 2. Figure 1. Oct 15, 2019 · Yet, pure ethyl lactate proved to be the most selective solvent to extract caffeine from green tea leaves, minimizing the co-extraction of catechins, with a caffeine/catechins selectivity of 2. cup. In the first step we have to brew a very strong cup of tea. 0 mL of the prepared tea. 1039/9781849739634-00030. Apr 13, 2024 · this reason, it seems reasonable to develop a method for determining caffeine in tea that is consistent with the principles of green chemistry and green chromatography. Isolation was done by liquid-liquid extraction using | Find, read and cite all the research you need Products containing caffeine include coffee, tea, soft drinks ("colas"), energy drinks, other beverages, chocolate, caffeine tablets, other oral products, and inhalation products. 2 PRINCIPLE O r CH, Caffeine Tea, coffee and cola nuts are the most common and rich sources of caffeine. Jul 3, 2019 · Use this procedure to extract and purify caffeine from tea leaves using solvent extraction to isolate and purify caffeine. Commercially, caffeine is obtained as a by-product from the decaffeinating process of coffee. pptx), PDF File (. 01 This study extracted caffeine from tea leaves (Thea sinensis) through filtration, multiple extractions with dichloromethane and sodium hydroxide, and evaporation. [45][12] However, caffeine has several side effects and drawbacks. f. The method was found to be fast, simple, cost effective and environmental friendly for the determination of caffeine in soft drinks with satisfactory results. The extracted sample contains caffeine and it is adsorbed on MgO. Table 1: Amount Jan 1, 2018 · Extraction of caffeine from industrial tea waste is referred to as the most popular and effective method so far which is used by multiple tea factories to make profit (Shalmashi et al. Pregnant women, athletes and children Jan 1, 2020 · antipyretic preparation [7]. Beaker (500ml). People can easily Guarana. Guarana seeds contain about four times the amount of caffeine as that found in coffee beans. Published 2016. 04 3 Roses 0. Keep in mind that the actual caffeine content of a cup of coffee or tea can vary quite a bit. The icon is shown above - it's the To extract caffeine from tea powder using polar - nonpolar solvent extraction technique. Black tea — about 40 (range: 20 to 90) mg of caffeine. Find out just how much caffeine is in coffee, tea, soda and energy drinks. Factors such as processing and brewing time affect the caffeine level. Nov 9, 2023 · Coffee, tea, soda, energy drinks — caffeine comes in a variety of forms, but they all have one important thing in common: The caffeine in them can be incredibly difficult to quit. Amount of caffeine. edu CHEM 2125-007 March 20, 2018 Abstract: The intention of this lab was to learn how to isolate caffeine from tea leaves using the method of extraction. The solubility of caffeine is 22 mg/mL (25 °C) and 670 mg/mL (100 °C). Tea is the most commonly and widely used soft beverage in the household. An extraction is taking place each time coffee or tea is made. Discard the tea bags. Three tea bags weighing 6. Combine 100 mL of water and 5 g of sodium carbonate (Na2CO3) in 250-mL beaker, cover with a watch glass, and wrap snuggly with aluminum foil. (Use a NEW page for the tables). Anxiety. The results obtained by Bermejo and co-workers have exhibited the combination of ethyl lactate and water (25:75) was 1. 6 million tonnes in 2021 and is predicted to increase to 7. White tea — about 15 mg of caffeine. Cool the tea extract to room temperature. al . The mixture was then cooled to about 55oC - 60oC and using a Buchner funnel and a filter flask, a vacuum filtration was performed. 0. Its In this experiment, students will use an organic solvent to extract the organic components, namely caffeine, from tea. It has numerous pharmacological and physiological effects, including cardiovascular, respiratory, renal, and smooth muscle effects, as well as effects on mood, memory, alertness, and physical and cognitive Sep 8, 2017 · The use of supercritical fluid extraction to an extract of caffeine from green tea has exhibited a recovery between 52% to nearly 93% regarding mode and co-solvent . Place the tea bags in the boiling water and let them steep for 7 - 10 minutes with a watch glass Sep 1, 2009 · A new method using high pressure processing to extract caffeine from green tea leaves was studied. Caffeine and several other compounds will be extracted from an aqueous solution (concentrated black tea) using an extraction solvent that is immiscible with water. In book: Comprehensive Organic Chemistry Experiments for the Laboratory Classroom (pp. 13% extraction rate. In this experiment, the caffeine was successfully extracted from a 2. The dichloromethane solution is then dried and evaporated, leaving behind crude caffeine. It is also increases the capacity of thinking. If you have problems with your PC locking or going to sleep, caffeine will keep it awake. Too much caffeine can also worsen symptoms of these disorders by intensifying feelings of worry, causing racing thoughts, increasing heart rate, and preventing relaxation and good-quality sleep. This secondary metabolite is regarded as a chemical defense because it has antimicrobial activity and is considered a natural insecticide. Drain the bottom layer into a conical flask because now the caffeine is extracted into the dichloromethane layer. Research has been taken to extract it from natural source more economically. Set up the extraction device from bottom to top, weigh 80mL 95% ethanol, add a half to the fat extractor (the liquid level can not exceed the highest point of the siphon), the rest is added to the three-necked bottle Determination of Caffeine in Different tea Samples. We have elaborated the science behind each step involved for your clearer understanding of the operations. p. INTRODUCTION: Tea is one of the most commonly used caffeinated beverages in the world. Anhydrous calcium chloride pellets were used to dry the solution and Oct 1, 2017 · The present study investigated the sequential extraction of caffeine and catechins from green tea biomass using an optimized SFE method. Using the proper extraction methods, the caffeine within a tea bag could potentially be isolated to yield a pure solid; the mass of this solid would reflect the actual yield of caffeine in the tea. 0 g of tea leaves to around 100 mL of water in a 400-mL beaker (cut open Prevent your computer from going to sleep. 196 grams were used. In a separatory funnel, the cooled tea/sodium carbonate mixture was added, followed by 3 rounds of 20mL of ethyl acetate. In fact, a wide variety of solvents including water, water in combination with organic solvents, methylene chloride, ethyl acetate, and supercritical carbon dioxide are used commercially to extract caffeine Aug 16, 2023 · For example, caffeine intoxication produces symptoms that can easily be confused with anxiety disorders, such as panic attacks. Extraction is the physical process by which a compound (or mixture of compounds) is transferred from one phase to another. Procedure: Extracting the caffeine (and other molecules) from tea leaves. Caffeine is found in many drinks such as coffee, tea, soft drinks and energy drinks. Black tea decaf — about 2 to 10 mg of caffeine. Caffeine is soluble in water and a variety of organic solvents, and both can be used to extract caffeine from tea leaves or coffee beans. To do so, caffeine must be introduced to a The purpose of this study was to investigate obtaining of caffeine from tea plant wastes by supercritical carbon dioxide extraction. Supercritical fluid extraction methods to extract caffeine from tea leaves are also known. Set up BLANK DATA tables for recording any numerical values AND in-lab observations. organic layer. Theory Caffeine The most important methylated alkaloid that occurs naturally is caffeine. Weight of teas 20. Apr 26, 2022 · Caffeine is shown in milligrams (mg). 0703 grams of caffeine was obtained, yielding a 1. The nitrogen present controls solubility. Add about 4 g of sodium carbonate. 0 g for the mass of tea and obtain 50. /sample of 50 gm. A mixture of Two Tea bags with 50mL and 2. Bring about 100 ml of water to boil in a 150 ml beaker. 47. This is why the tea was boiled with water, because the caffeine is soluble in it. Group A performs a traditional solvent extraction using methylene chloride (CH 2 Cl 2)/alkaline water and group B using a SFE method. Cap the tubes and gently shake for several seconds, carefully vent the tubes to release any pressure buildup by slowly opening the caps. Dec 31, 2021 · extraction of caffeine from tea in hot water for 15 minutes, while 10 minutes. Jan 1, 2014 · The aim of this study was to determine the content of caffeine in five types of tea (white, yellow, green, oolong, black) and two types of maté tea (green maté and roasted maté tea). 10) until the water is boiling. Caffeine is first extracted from tea bags into dichloromethane through repeated extraction. Experiments were carried out with tea stalk and fiber wastes of Turkish tea plants that has no economical value. Make sure you also REFERENCE. 1. Similarly, world tea consumption increased from 6. DOI: 10. 3. It works by blocking the effects of adenosine, a brain chemical that makes you feel tired. The solution of these dissolved compounds is referred to as the desired extract. In such cases, ethanol [29,30] or isopropanol [31] is added to carbon dioxide as an View PDF. As stated in Chapter 1, caffeine is the most widely used central nervous system (CNS) stimulant in the world. Louis; Chemistry) 3: Extraction of Caffeine (Experiment) is shared under a license and was authored, remixed, and/or curated by LibreTexts. Transfer the tea extract from the 50 ml Erlenmeyer flask to a 125 ml separatory cellulose – is not. pdf), Text File (. So use these numbers as a guide. [4] Some drinks containing extracts of these seeds can contain up to 125 mg caffeine per serving. extraction of caffeine from natural source is more. An acid/base liquid-liquid extraction took place in order to force caffeine into the. The goal was to demonstrate the process of isolating a Enter 8. Brewed coffee 80-135 mg Brewed tea 40-60 mg @ extract caffeine from tea leaves 0 purify the sample so obtained, and @ outline the importance of caffeine. Use hot water to extract the caffeine from the tea leaves. Caffeine occurs in tea, coffee, guarana, maté, kola nuts, and cacao. Aug 6, 2014 · This exhaustive review of various extraction parameters, decaffeination process of tea and large scale cost effective isolation of tea components with aid of modern technology can assist people to This work will focus on the procedure for caffeine extraction and their working principles and various analytical methods for its separation and detection. 30-34) Edition into the 50 ml Erlenmeyer flask. Pharmacology of Caffeine. While the tea bags are cooling add 2. This gave calculated values of 59. Bring in two tea bags from home. 2920 g Weight of (round bottom flask + crude caffeine) 113. Natural products-An Indian journal, 10(1), 27-29. It works by simulating a keypress once every 59 seconds, so your machine thinks you're still working at the keyboard, so won't lock the screen or activate the screensaver. stockton. 05 g of tea leaves along with approximately 55 mg of caffeine [1] . May 1, 2015 · PDF | Caffeineextracted and characterised from tea (black) leaves and coffee beans. Botanical sources of caffeine include kola nuts, guarana, and yerba mate. Green tea contains 30-50 mg of caffeine per cup. The solvents used in the experiment were an aqueous sodium carbonate and dichloromethane (DCM). This is a seed from a South American plant that is processed as an extract in foods, energy drinks, and energy supplements. Cover the mouth of the conical flask to avoid evaporation of the solution. The intention of this lab was to learn how to isolate caffeine from tea leaves using the method of extraction. Table No-2: Caffeine Content in Tea/Coffee Sample (Extraction with water) TEA/COFFEE SAMPLES AMOUNT OF CAFFEINE (gm) Brook Bond Red Label 0. 26g tea bag was 0. fj pf uj gy ei rr ju vu cp ja